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. 2006 Jan;188(1):103–114. doi: 10.1128/JB.188.1.103-114.2006

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Copyright © 2006, American Society for Microbiology

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FIG. 2. — (A) Motility assay of S. enterica serovar Typhimurium fliA-defective strain TH4387 with the following plasmids: vector (pBAD24), P_araB-σ²⁸ S. enterica serovar Typhimurium (S.t.) (pMC147), P_araB-σ²⁸ A. aeolicus (A.a.) (pJK558), and P_araB-σ²⁸ C. trachomatis (C.t.) (pJK629). (B) Western analysis of total cellular proteins from S. enterica serovar Typhimurium hybrid strains fliA (σ²⁸) from A. aeolicus and C. trachomatis probing with anti-FliC antiserum. Lane 1, TH437 (S. enterica serovar Typhimurium wild-type strain LT2); lane 2, TH6827 (ΔfliA5805::tetRA); lane 3, TH8142 ((fliA6068 [A. aeolicus fliA]); lane 4, TH8982 ((fliA6325 [C. trachomatis rspD]). Fourfold more cellular lysate was loaded in lanes 2 to 4 than in lane 1.