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. 2006 Jan;188(1):103–114. doi: 10.1128/JB.188.1.103-114.2006

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Copyright © 2006, American Society for Microbiology

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FIG. 4. — β-Galactosidase activities of lac gene transcriptional fusion reporters to σ²⁸-dependent genes motA and fliC in S. enterica serovar Typhimurium. The σ²⁸ coding sequences were replaced by λ-Red recombination with coding sequences from either σ²⁸ from A. aeolicus (fliA) or C. trachomatis (rspD). The flgM coding sequence was replaced with A. aeolicus flgM. C. trachomatis RsbW, putative negative regulator of RspD, was encoded in trans by plasmid pJK627. (A) MotA-lac β-galactosidase activities were compared relative to those of the following isogenic WT σ²⁸ strains (WT = 100%): TH6238, black bars; TH8196, gray bars; TH9033, white bars. Lane 1, ΔfliA, ΔflgM (TH8197); lane 2, S. enterica serovar Typhimurium (S.t.) fliA, S. enterica serovar Typhimurium flgM (TH6238); lane 3, S. enterica serovar Typhimurium fliA, ΔflgM (TH8194); lane 4, S. enterica serovar Typhimurium fliA, A. aeolicus (A.a.) flgM (TH8120); lane 5, ΔfliA, ΔflgM (TH8197); lane 6, A. aeolicus fliA, A. aeolicus flgM (TH8122); lane 7, A. aeolicus fliA, ΔflgM (TH8196); lane 8, A. aeolicus fliA, S. enterica serovar Typhimurium flgM (TH8059,); lane 9, ΔfliA, ΔflgM (TH8197); lane 10, C. trachomatis (C.t.) rspD, C. trachomatis rsbW (TH9031); lane 11, C. trachomatis rspD, ΔflgM (TH9032); lane 12, C. trachomatis rspD, S. enterica serovar Typhimurium flgM (TH9033). (B) FliC-lac β-galactosidase activities were compared relative to those of the following isogenic WT σ²⁸ strains (WT = 100%): TH4098, black bars; TH8125, gray bars; TH9040, white bars. Lane 1, ΔfliA, ΔflgM (TH8201); lane 2, S. enterica serovar Typhimurium fliA, S. enterica serovar Typhimurium flgM (TH4098); lane 3, S. enterica serovar Typhimurium fliA, ΔflgM (TH8198); lane 4, S. enterica serovar Typhimurium fliA, A. aeolicus flgM (TH8123); lane 5, ΔfliA, ΔflgM (TH8201); lane 6, A. aeolicus fliA, A. aeolicus flgM (TH8125); lane 7, A. aeolicus fliA, ΔflgM (TH8200); lane 8, A. aeolicus fliA, S. enterica serovar Typhimurium flgM (TH8061); lane 9, ΔfliA, ΔflgM (TH8201); lane 10, C. trachomatis rspD, C trachomatis. rsbW (TH9040); lane 11, C. trachomatis rspD, ΔflgM (TH9041); lane 12, C. trachomatis rspD, S. enterica serovar Typhimurium flgM (TH9042).