TABLE 2.

Summary of mutants isolated in A. aeolicus fliA

Strain	Mutation relative to the start codon	Comments	Change in % of codon frequency from WT to mutant	Relative activity (P value) compared toa:
				TH8125 in the presence of FlgMb	TH8200 in the absence of FlgMc
TH8125		WT		1
TH8200		ΔflgM			1
TH8612	C→T +10 bp	CCT→TCT P4S, domain σ2	7.8 to 8.5	2.8 (<0.001)	1.2 (>0.05)
TH8613	A→T +79 bp	ACA→TCA T27S, domain σ2	4.7 to 5.9	1.7 (0.012)	1.6 (<0.001)
TH8614	A→G +87 bp	ATA→ATG, 129M, domain σ2	4.2 to 23.6	1.4 (0.006)	1.6 (0.003)
TH8615	A→G +445 bp	AGA→GGA, R149G, domain σ3-4 linker	2.2 to 6.3	1.3 (0.032)	0.8 (0.042)
TH8616	C→T +524 bp	ACG→ATG, T175M, domain σ3-4 linker	17.1 to 23.6	2.9 (<0.001)	1.6 (0.013)
TH8617	A→G +566 bp	GAA→GGA, E189G, domain σ4	55 to 14.5	1.3 (0.030)	1.1 (>0.05)
TH8618	A→G +596 bp	GAA→GGA, E199G, domain σ4	55 to 14.5	1.4 (0.016)	1.2 (>0.05)

^aRelative β-galactosidase activities were calculated from Fig. 5B.

^bP values were determined using two-tailed t tests on β-galactosidase activities compared to TH8125.

^cP values were determined using two-tailed t tests on β-galactosidase activities compared to TH8200.