TABLE 3.

Summary of mutations isolated in A. aeolicus flgM

Strain	Mutation relative to the start codon	Commentsa	Change in % of codon frequency from WT to mutant	% fliC-lac β-galactosidase activity relative to WT	Relative activityb
TH8125		WT		100	1
TH8200		ΔflgM		395	4.0
TH8604	A→G −6 bp	RBS		116	1.2
TH8605	A→C −5 bp	RBS		316	3.2
TH8606	A→G −4 bp	RBS		95	0.95
TH8607	A→G +1 bp	ATG→GTG M1V	26.3 to 26.4	168	1.7
TH8608	A→G +56 bp	AAG→AGG K19R, rare codon	11.7 to 1.2	116	1.2
TH8609	ΔA +55-56 bp	Frameshift after codon 18		395	4.0
TH8610	ΔA +177 bp	Frameshift after codon 59		374	3.7
TH8611	ΔA +199 bp	Frameshift after codon 67		437	4.4

^aRBS, ribosome binding site.

^bRelative activity calculated from β-galactosidase activities in column 5.