FIG. 1.

Generation of gtf gene disruptants. (A to D) Schematic diagram of each gtf region on the chromosome of the wild-type M. smegmatis mc²155 strain (Wt) and its gene disruptants Δgtf1, Δgtf2, Δgtf3, and Δgtf4. The shaded boxes indicate the regions included in recombinant phage for gene disruption. The black arrows represent the coding region of each gtf gene. The gray boxes represent the hygromycin resistance cassette (hyg). The primers used for PCR analysis are indicated by small arrows. (E) PCR analyses of the wild type and each disruptant using the primers indicated above.