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TLC analyses of crude GPL extracts from the M. smegmatis mc2155 strain (Wt) and its gene disruptants transformed with M. avium gtfA and gtfB. Total lipid fraction after mild alkaline hydrolysis was spotted on plates and developed in CHCl3-CH3OH (9:1 [vol/vol]). GPLs were visualized by spraying with 10% H2SO4 and charring. Each total lipid fraction was extracted from an equal weight of harvested cells.
