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SDS-PAGE analysis of AidB purification. AidB was isolated from extracts of E. coli C41[DE3] cells containing pET28aAidB by ammonium sulfate precipitation followed by successive chromatography using phenyl-Sepharose and DEAE-Sepharose columns. Fractions were analyzed by using SDS-10% PAGE. Lane 1, standards; lane 2, cell extracts; lane 3, 30 to 45% ammonium sulfate pellet dissolved in buffer A; lanes 4 and 5, phenyl-Sepharose fractions; lane 6, DEAE-Sepharose pooled fractions.
