TABLE 7.

Expression ratios of genes specifically down-regulated in at least three clinical SCVs

ORF (COL)	Gene	Description	Known modulation and characteristics	Reference	Fold changea
					hemB	CF03	CF06	CF07	CF10
Virulence-associated genes
478		Exotoxin 3			6.6	−3.6	−2.3	−6.4b	−6.2
1168	fib	Fibrinogen-binding protein			3.0	−2.5	−1.6	−2.2	−2.6
1173	hla	α-Hemolysin	Up-regulated by Agr; down-regulated by SigB	9	1.8	−1.6	−2.4	−2.2b	−4.3
Biosynthesis/transport/metabolism-associated genes
688	mntC	Manganese ABC transporter, ATP-binding protein			−1.4	−1.8	−2.9	−8.1	−2.3
689	mntB	Manganese ABC transporter, ATP-binding protein			−1.5	−1.6	−2.5	−6.4	−2.1
1052	menD	Carboxylate synthase			−1.2	−2.8	−1.8	−5.0	−3.1
1140	isdA	Heme transport, cell wall LPXTG protein	Down-regulated in biofilm	7	1.9	−2.8	−1.7	−2.5	−2.1
2166	htsB	Heme transport ABC transporter, permease			−1.0	−2.9	−1.5	−2.7	−2.3

^aExpression ratios (SCV test strain/Newbould, both strains in the early exponential phase of growth) showing a ≥2-fold decrease in at least three clinical SCVs (but not hemB) are listed. A decrease is indicated by a minus sign.

^bCalculated with a biological duplicate, not a triplicate like the other values.