TABLE 5.
Rates of germination of gerB* spores with amino acids with or without glucosea
Strain (receptor[s] present) | Rates of spore germination with indicated germinant and glucoseb
|
|||||||
---|---|---|---|---|---|---|---|---|
l-Alanine
|
l-Asparagine
|
l-Serine
|
l-Threonine
|
|||||
No | Yes | No | Yes | No | Yes | No | Yes | |
FB22 (GerBA* GerK) | 31 | 74 | 45 | 100c | 26 | 48 | 4 | 12 |
PS3710 (GerBA*) | 29 | 28 | 36 | 35 | 28 | 30 | 4 | 3 |
PS3521 (GerBB* GerK) | 32 | 76 | 40 | 82 | 27 | 52 | 12 | 27 |
PS3665 (GerBB*) | 36 | 36 | 39 | 40 | 25 | 23 | 12 | 11 |
Spores of various strains were germinated with amino acids (10 mM l-alanine, 6 mM l-asparagine, 30 mM l-serine, or 30 mM l-threonine) with or without 10 mM glucose, and the rates of spore germination were measured and calculated as described in Materials and Methods.
Rates of spore germination are given relative to the value for FB22 spores germinated with l-asparagine plus glucose, which was set at 100. This value is equivalent to 100% spore germination in 22 min. “No” and “Yes” refer to the absence and presence, respectively, of glucose. Boldface type indicates that glucose stimulates the germination of spores via GerB*s with l-alanine, l-asparagine, l-serine, and l-threonine by ∼2-fold. Italic type indicates that the effect of glucose was not dependent on GerA but required GerK.
Value set at 100.