TABLE 2.

Oligonucleotide primers

Name	5′ → 3′ sequencea	Purpose
JG489	ATGCGAATTTTACTGGTAGAAGATGACACAGTGTAGGCTGGAGCTGCTTCG	λ-Red deletion of preA or preAB
JG490	TCATGCGTCACCCAGGGTGTAGCCGATGCCCATATGAATATCCTCCTTAG	λ-Red deletion of preA
JG557	ATGAAATTGACGCAACGTCTCAGCCTGACAGTGTAGGCTGGAGCTGCTTCG	λ-Red deletion of preB
JG558	TCGGTACGCCTTTGGCGTCGAGCGGCGTTTCATATGAATATCCTCCTTAG	λ-Red deletion of preB or preAB
JG634	ATAAGAATGCGGCCGCAGTTGATGTTGTTCGC	SOE-PCR preB[H246G]
JG635	CCGACGCCGCCGGCGAATTG	SOE-PCR preB[H246G]
JG636	CAATTCGCCGGCGGCGTCGG	SOE-PCR preB[H246G]
JG637	GAATTCTCCGCGGCGTTGCCAAACGA	SOE-PCR preB[H246G]
JG708	GGAATTCATGCGAATTTTACTGG	Amplify preAB
JG709	GGGGTACCTTACCAACTTACTACGGC	Amplify preAB
JG795	CGGGCAGCCCTAAAGCCTGCACCATCAGA	SOE-PCR pmrA[D51A]
JG796	TCTGATGGTGCAGGCTTTAGGGCTGCCCG	SOE-PCR pmrA[D51A]
JG797	CGCGTCGACTATTACAACCGTTATCCACCG	SOE-PCR pmrA[D51A]
JG798	GCTCTAGAGTACTGATTAACTGGAACACC	SOE-PCR pmrA[D51A]
JG1055	CCGGAATTCCGCGAGTTACCGCAAGGAAGAACAGATGCG	Amplify preA
JG1056	CCGGAATTCTCATGCGTCACCCAGGGTGTAGCCGATGC	Amplify preA
JG1190	CTTATGATGCGGTTATTTTAGCGTTGACGCTGCCAGGC	Mutagenize preA[D51] to A
JG1191	TAAAATAACCGCATCATAAGGCGCGCTGTAAAGCG	Mutagenize preA[D51] to A

^aRestriction enzyme sites are underlined; P1 and P2 priming sites in pKD3 and pKD4 are in italics.