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Photomicrographs of F. johnsoniae colonies. Colonies were grown for 30 h at 25°C on PY2 agar media. Photomicrographs were taken with a Kodak DC290 digital camera mounted on an Olympus IMT-2 inverted microscope. Bar indicates 0.5 mm. A, Wild-type F. johnsoniae MM101 with pCP23. B, secDF mutant CJ974. C, CJ974 complemented with pSN2, which carries secDF.
