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Expression of mutant EmhBHis proteins in P. fluorescens cLP6a-1 relative to EmhBHis expression. Cell extracts (10 μg total protein) from P. fluorescens cLP6a-1 carrying the mutant EmhBHis proteins were separated by SDS-PAGE and subjected to immunoblotting with an antibody against the histidine tag. The proteins are indicated above the lanes.
