TABLE 2.
Conservation of amino acid residues mutated in EmhB in RND multidrug and solvent efflux pumpsa
| Efflux pumpc | Mutated residue in EmhBb
|
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Central cavity
|
Pore domain
|
Outer membrane protein docking domain
|
|||||||||||||
| A384 | A385 | F386 | F458 | F459 | N99 | D101 | Y157 | F316 | F317 | F682 | F281 | F325 | N282 | A206 | |
| ArpB | A | A | A | F | F | N | D | Y | F | F | F | F | F | N | A |
| TtgB | A | A | A | F | F | N | D | Y | F | F | F | F | F | N | A |
| MexB | A | A | F | F | F | D | D | Y | F | M | F | F | Y | N | A |
| SrpB | P | Y | F | F | F | D | D | L | Y | L | Y | Y | Y | K | A |
| TtgH | P | Y | F | F | F | D | D | L | Y | L | Y | Y | Y | K | A |
| TtgE | P | Y | F | F | F | N | D | L | Y | L | Y | Y | Y | R | A |
| AcrB | A | A | F | F | F | D | D | Y | F | F | E | F | Y | N | A |
| MexD | Y | L | L | F | M | D | D | Y | F | F | R | L | V | N | A |
| MexY | L | G | L | F | F | N | D | I | Y | F | R | V | I | N | A |
a
Protein sequences were aligned by using ClustalX (28).
b
Residues that are conserved in EmhB are indicated in boldface type.
c
ArpB (GenBank accession number AAF73832 [12]) and SrpB (AAD12176 [13]) from P. putida S12; TtgB (AAC38671 [24]), TtgH (AAK69564 [25]), and TtgE (CAB72259 [17]) from P. putida DOT-T1E; MexB (NP_249117 [14]), MexD (NP_253288 [22]), and MexY (NP_250708 [1]) from P. aeruginosa; and AcrB (NP_414995 [19]) from E. coli.