TABLE 1.
Bacteral strains
| Strain | Genotype | Source or reference(s) |
|---|---|---|
| E. coli | ||
| DH5α | supE44 ΔlacU (φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Laboratory stock |
| DW1030/RK231 | ΔlacX74 rpsE gyrA100 recA13 thi/Knr Tcr | 17, 55 |
| JM107 | F′ traD36 lacIq Δ(lacZ)M15 proA+B+/e14−(mcrA) Δ(lac-proAB) thi gyrA96 (Nalr) endA1 hsdR17 (rK− mK+) relA1 supE44 | 62 |
| B. subtilis | ||
| AF21 | ΔamyE::Φ(citBp21-lacZ cat) | 15 |
| HKB165 | ΔamyE::Φ(citBp21-lacZ cat) citB::spc | 26 |
| HKB166 | ΔamyE::Φ(citBp21-lacZ cat) ΔcitA::neo ΔcitZ471 citB::spc | 26 |
| HKB186 | ΔamyE::Φ(citBp21-lacZ cat) ΔccpCBs::ble | AF21 × ΔccpCBs::ble |
| HKB187 | ΔamyE::Φ(citBp21-lacZ cat) ΔccpCBs::ble citB::spc ΔcitA::neo ΔcitZ471 | HKB166 × ΔccpCBs::ble |
| HKB199 | ΔamyE::Φ(citBp21-lacZ cat) ΔccpCBs::ble citB::spc | HKB186 × DNA HKB165 |
| HKB218 | ΔamyE::Φ(citBp21-lacZ cat) ΔccpCBs::ble Ω(PccpC-ccpCLm erm) | HKB186 × pHK99 |
| HKB219 | ΔamyE::Φ(citBp21-lacZ cat) ΔccpCBs::ble citB::spc ΔcitA::neo ΔcitZ471 Ω(PccpC-ccpCLm erm) | HKB187 × pHK99 |
| HKB220 | ΔamyE::Φ(citBp21-lacZ cat) ΔccpCBs::ble citB::spc Ω(PccpC-ccpCLm erm) | HKB199 × pHK99 |
| L. monocytogenes | ||
| EGDe | Animal isolate; genome sequencing strain | 16 |
| HKB213 | Δint::Φ(lmo0847′-lacZ neo) | EGDe × pHK85 |
| HKB214 | Δint::Φ(citBLm′-lacZ neo) | EGDe × pHK86 |
| HKB216 | Δint::Φ(lmo0847′-lacZ neo) ΔccpC::spc | HKB213 × pHK95 |
| HKB217 | Δint::Φ(citBLm′-lacZ neo) ΔccpC::spc | HKB214 × pHK95 |