TABLE 3.
Complementation of an L. monocytogenes ccpC mutanta
| Strain | Relevant genotype | Fusion | β-Galactosidase activityb |
|---|---|---|---|
| HKB214 | ccpC+ | citBLm-lacZ | 8.0 |
| HKB217 | ΔccpCLm::spc | citBLm-lacZ | 18.6 |
| HKB217(pEMM18) | ΔccpCLm::spc (pEMM18 [ccpC+]) | citBLm-lacZ | 11.5 |
| HKB217(pHP13) | ΔccpCLm::spc (pHP13) | citBLm-lacZ | 19.4 |
a
Cultures were grown at 30°C in HTM medium supplemented with hemin (0.2 μg/ml) and glutamine (1.6 mg/ml). Samples were taken in mid-exponential growth phase (OD600 = 0.21 to 0.25) for β-galactosidase assays.
b
Miller units. Values are the averages for at least two experiments, and variation was less than 10%.