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. 2006 Jan;26(1):324–333. doi: 10.1128/MCB.26.1.324-333.2006

FIG. 1.

FIG. 1.

(A) Sequences of the TFOs and of the duplex containing the 16-bp (underlined) triplex target site used in this study. The experimental construction for cell experiments is shown: an insert of 54 bp, containing the original triplex target (plasmid pWT), was cloned within the 5′ transcribed but untranslated region of the firefly luciferase gene (luc), under the control of the SV40 promoter, between the HindIII and NcoI sites of vector pGL3Pr. The 54-bp region around the triplex site is shown. The region on the 3′ side of the triple helix, i.e., the 3′ side of the oligopurine strand of the duplex, contains two Topo I-mediated cleavage sites, b and c, observed in the presence of free CPT. All Topo I cleavage sites observed in vitro are indicated by letters. M, 5-methyl-2′-deoxycytidine; P, 5-propynyl-2′-deoxyuridine. The 16-nt sequence of the HIV-CPT(10) conjugate used as a control is shown. (B) Structures of the CPT conjugate derivatives used in this study.