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. 2006 Jan;26(1):371–380. doi: 10.1128/MCB.26.1.371-380.2006

FIG. 3.

FIG. 3.

Overexpression of TUP1 specifically stabilizes Deg1-containing proteins. (A) Pulse-chase analysis of Deg1-β-gal and Deg1-Ura3 degradation in JY104 cells transformed with the empty GAL1 expression vector (vector) or with TUP1 expressed from this vector (↑TUP1). Deg1-containing proteins were immunoprecipitated from lysates with antibodies to α2 and quantitated by PhosphorImager analysis. (B) The degradation kinetics of β-gal-SL17 in vector-containing JY102 cells and in cells overexpressing TUP1. Radiolabeled β-gal-SL17 was immunoprecipitated from lysates with antibodies to β-gal and quantitated with a PhosphorImager.