TABLE 2.

Overexpressed cDNA clones impair deg1-mediated degradation

Gene	No. of isolated clones/no. of different cDNAs	Increase in Deg1-β-gal activity (fold)	Comment(s)
Full-length cDNAs
GLC7	1/1	2
SKP1	1/1	2
SMI1	1/1	2
SRL1	1/1	2
TUP1	2/2	7
UBC4	1/1	2
UBC6	20/6	2-4
YDR132c	1/1	2
cDNA fragments or inverted cDNAs
“EFB1”	2/1	2	cDNA in inverted orientation
“HEX3”	1/1	2	Fragment from 5′ end of gene; predicted to encode the first 269 amino acids of the 619-residue wild-type protein
“MNR2”	7/1	3	Fragment from 3′ end of gene; 5′ end of cDNA starts at +2258; predicted to encode a 22-amino-acid peptide from the +1 reading frame
“SAS3”	3/1	2	cDNA in inverted orientation
“SIS2”	2/1	3	cDNA in inverted orientation; predicted to encode an 118-amino-acid polypeptide
“STE12”	8/1	6	Fragment from 3′ end of gene; 5′ end of cDNA starts at +825; predicted to encode a 63-amino-acid polypeptide from the +1 reading frame
“YHR054c”	3/1	3	Fragment from 3′ end of gene; 5′ end of cDNA starts at +467