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. 2006 Jan;26(1):28–38. doi: 10.1128/MCB.26.1.28-38.2006

FIG. 3.

FIG. 3.

Inhibition of SIRT1 catalytic activity enhances p53 lysine 382 acetylation after a variety of DNA-damaging agents and is not cell type specific. (A) NCI-H460 cells were treated with adriamycin, hydroxyurea, or hydrogen peroxide for 6 h. p53 was immunoprecipitated and immunoblotted with an anti-acetylated p53 Lys 382 antibody or p53 antibody (Ab-7). (B) U-2 OS and MCF-7 cells were treated with etoposide in the presence or absence of 1 μM EX-527 for 6 h. Immunoprecipitation and immunoblotting was performed as described for panel A. (C) HMEC were treated with etoposide or adriamycin in the presence or absence of 1 μM EX-527 for 6 h. Immunoprecipitation and immunoblotting of p53 was performed as described for panel A. Total SIRT1 levels were measured by immunoblotting using antibody 07-131 (Upstate Cell Signaling Solutions). +, present; −, absent.