FIG. 7.
Effect of inhibiting SIRT1 and HDAC1 on p53 lysine 382 acetylation and cell growth. (A) NCI-H460 cells were treated for 6 h with various concentrations of TSA and 1 μM EX-527 in the presence of 20 μM etoposide. Cell lysates were analyzed by immunoblotting with an anti-acetylated p53 Lys 382 antibody or p53 antibody (Ab-7). The graph shows the quantification of bands on the gel shown, but this is representative of the results from two such experiments. +, present; −, absent. (B to E) NCI-H460 cells were treated with increasing concentrations of etoposide or adriamycin and deacetylase inhibitors as indicated. Dimethyl sulfoxide (DMSO) was used as the vehicle control. (B and D) Cell proliferation at 72 h was measured by monitoring [14C]thymidine incorporation; (C and E) cell viability was measured at 48 h.