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Effect of overexpression of SIRT1 on hydrogen peroxide-induced cell death. Cell viability was measured in IMR-90 (A) or MCF-7 (B) cells overexpressing SIRT1 or GFP treated with increasing concentrations of hydrogen peroxide for 24 h. Overexpression of SIRT1 in these cells was confirmed using immunoblot analysis (insets). Endogenous SIRT1 is not visible because very small amounts of protein (2 to 4 μg) were needed to obtain a clean signal from cells overexpressing SIRT1 protein.
