FIG. 6.

TRAF6 recruitment to endosomal IL-1R1 is dependent on H₂O₂. (A) MCF-7 cells were infected with AdDyn(DN) or AdBglII (negative control). At 48 h postinfection, cells were treated with IL-1β at either 4°C (1 h) or 37°C (20 min). IL-1R1 was then immunoprecipitated, followed by Western blotting for the indicated proteins. (B) Cells were treated with IL-1β at 4°C (1 h) or 37°C (20 min) in the presence or absence of H₂O₂. IL-1R1 was then immunoprecipitated, followed by Western blotting for the indicated proteins.