FIG. 3.

SRm160 and Sam68 coimmunoprecipitate. 293T cells were transfected with plasmids containing either Flag-tagged SRm160 (A) or HA-tagged Sam68 (B) or corresponding empty vectors. Cell lysates were immunoprecipitated using anti-Flag or anti-HA antibodies, respectively (indicated at the top of the gels). Total cell lysates (input) as well as immunoprecipitants (IPs) were analyzed by SDS-polyacrylamide gel electrophoresis. Western blot analyses were performed using antibodies of Sam68 (A, Flag IP) or SRm160 (B, HA IP). The positions of Sam68 and SRm160 are indicated on the right of the gels.