FIG. 5.

Knockdown of SRm160 reduces tumor cell invasiveness. (A) Quantitative RT-PCR analysis of the v5 mRNA from HeLa cells transiently transfected with either control siRNA or v5 siRNA. Primers specific to v5 were used. Amplification of DHFR mRNA was used as an internal control. (B) Invasion assays. HeLa cells were transfected with control, CD44 v5, or SRm160 siRNA for 48 h. These cells were then suspended in serum-free medium and placed in an invasion chamber and induced to migrate toward serum-containing medium, requiring invasion through a matrigel-coated membrane. After 22 to 24 h, cells that invaded through the membrane were fixed, stained, and counted. Representative photographs of these cells are shown. (C) Quantitation of tumor cell invasion described in panel B. The percentage of cell invasion was normalized to that of cells transfected with control siRNA.