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. 2005 Dec 23;1(6):e79. doi: 10.1371/journal.pgen.0010079

Figure 4. Polymorphic Regulation of cen180 Transcripts.

Figure 4

Primers were designed to recognize sample cen180 cDNAs from dcl1 and met1 mutants of Landsberg erecta (see Figure 3C) and dcl3 and met1 mutants of Columbia. PCR amplification using genomic DNA templates (DNA) tested for the presence of the particular repeats in the genome of each ecotype. RT-PCRs using RNA detected transcripts in WT (wt) and mutants of each ecotype as indicated. The cen180 Fc + Rc primers served as positive controls in the presence of reverse transcriptase and negative controls in its absence. RNA was prepared from aerial tissues from 28-day-old plants.