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. 2005 Nov 8;392(Pt 1):221–229. doi: 10.1042/BJ20050425

Figure 4. Stage-specific expression of PfPDE1 transcripts.

Figure 4

In order to obtain a synchronous culture, ring form-rich parasites, prepared as described in the Experimental section, were cultured. After 21, 32, 38, 43 and 50 h, each sample was collected and the stage-specific cDNAs were prepared. The percentage of ring form, trophozoite, and schizont was calculated by counting the numbers of each cell form (upper panel). Expression levels of PfPDE1 transcripts (nt 1870–2655) and falcipain-3 (GenBank® accession number AF258791, nt 686–1528) were examined by RT-PCR. After separation on a 1.5% agarose gel, PCR products were detected with ethidium bromide staining (lower panel).