Table 1. Survival of mice after cell lineage-specific gene targeting of the RXRα floxed allele.
| Genotype
|
Survival, no. observed/no. expected | ||||||
|---|---|---|---|---|---|---|---|
| Strain | n | nCre+ | Age, days | Breeding | cre | RXRα | |
| tie2-cre | 56 | 39 | P20 | Tie2cre/+ RXRαf/+ × RXRαf/f | tie2 | RXRα f/+ | 22/14 |
| tie2 | RXRα f/f | 17/14 | |||||
| wnt1-cre | 97 | 66 | P20 | W1cre/+ RXRαf/+ × W1cre/+ RXRαf/+ | wnt1 | RXRα +/+ | 20/18 |
| wnt1 | RXRα f/+ | 27/36 | |||||
| wnt1 | RXRα f/+ | 19/18 | |||||
| tie2-cre mlc2V-cre | 57 | 46 | P20 | Tie2cre/+ RXRαf/f × M2vcre/+ RXRαf/f | tie2 | RXRα f/f | 13/14 |
| tie2 mlc2v | RXRα f/f | 10/14 | |||||
| mlc2v | RXRα f/f | 23/14 | |||||
| tie2-cre wnt1-cre | 21 | 15 | P20 | Tie2cre/+ RXRαf/f × W1cre/+ RXRαf/f | tie2 | RXRα f/f | 3/5 |
| tie2 wnt1 | RXRα f/f | 8/5 | |||||
| wnt1 | RXRα f/f | 4/5 | |||||
| g5-cre | 100 | 51 | E13 | G5cre/+ RXRαf/+ × RXRαf/f | g5 | RXRα f/+ | 26/25 |
| g5 | RXRα f/f | 25/25 | |||||
| g5-cre | 64 | 31 | E16 | g5 | RXRα f/+ | 20/16 | |
| g5 | RXRα f/f | 11/16 | |||||
| g5-cre | 43 | 18 | P20 | g5 | RXRα f/+ | 14/11 | |
| g5 | RXRα f/f | 4/11 | |||||
Animals hemizygous for either tie2-cre (endothelial-specific), wnt-1-Cre (neural crest-specific), a combination of tie2-cre and mlc2v-cre (ventricular myocyte-specific), a combination of tie2-cre and wnt-1-cre, or the epicardial-specific G5-cre were crossed with the RXRα floxed allele mouse to obtain each of the above cre animals that were also hemizygous for RXRα-flox allele. These animals were then bred to the homozygous RXRα-flox to obtain offspring with lineage-specific removal of RXRα. Survival rates and respective genotypes were scored. Genotypes corresponding to Cre- animals are not indicated. n, the total number of animals resulting from each breeding scheme; nCre+, the number of Cre positive animals, Pn, postnatal day n.