Table 1. Values for FRET ratios under various conditions.
| FRET ratio
|
||
|---|---|---|
| Constructs | Confocal | CCD |
| CFP + YFP* | 0.94 ± 0.03 (n = 36) | 0.97 ± 0.03 (n = 34) |
| α2A-CFP + KCNQ1-YFP | 0.90 ± 0.04 (n = 19) | 0.90 ± 0.02 (n = 24) |
| D2-CFP + M4-YFP* | 0.94 ± 0.05 (n = 14) | ND |
| α2A-CFP + α2A-YFP | 1.54 ± 0.06 (n = 34, P < 0.001) | 1.72 ± 0.06 (n = 29, P < 0.001) |
| M4-CFP + M4-YFP | 1.88 ± 0.15 (n = 6, P < 0.001) | 2.02 ± 0.12 (n = 12, P < 0.001) |
| Go-CFP + α2A-YFP | 1.72 ± 0.07 (n = 28, P < 0.001) | ND |
| Go-CFP + α2A-YFP + Gβ1 + Gγ2 | 1.41 ± 0.07 (n = 13, P < 0.01) | 1.66 ± 0.12 (n = 11, P < 0.001) |
| Gs-CFP + α2A-YFP | 1.18 ± 0.03 (n = 13, NS) | ND |
| Gs-CFP + α2A-YFP + Gβ1 + Gγ2 | ND | 1.06 ± 0.05 (n = 19, NS) |
| α2A-CFP + Gβ1-YFP + Gγ2 + Go | 1.54 ± 0.08 (n = 19, P < 0.001) | 1.90 ± 0.09 (n = 22, P < 0.001) |
| α2A-YFP + Gβ1 + Gγ2-CFP + Go | ND | 1.69 ± 0.12 (n = 6, P < 0.001) |
| Go-CFP + M4-YFP | 2.08 ± 0.17 (n = 11, P < 0.001) | ND |
| M4-CFP + Gβ1-YFP + Gγ2 + Go | 1.66 ± 0.07 (n = 12, P < 0.001) | 1.51 ± 0.05 (n = 18, P < 0.001) |
| M4-YFP + Gβ1 + Gγ2-CFP + Go | ND | 1.84 ± 0.20 (n = 8, P < 0.001) |
| Go-CFP + A1-YFP | 1.59 ± 0.09 (n = 19, P < 0.001) | ND |
| A1-CFP + Gβ1-YFP + Gγ2 + Go | 1.48 ± 0.10 (n = 13, P < 0.001) | 1.50 ± 0.05 (n = 18, P < 0.001) |
| A1-YFP + Gβ1 + Gγ2-CFP + Go | 1.67 ± 0.17 (N = 6, P < 0.001) | 1.53 ± 0.09 (n = 12, P < 0.001) |
| D2S-YFP + Go-CFP | 1.55 ± 0.08 (N = 16, P < 0.001) | ND |
| D2S-YFP + Go-CFP + Gβ1 + Gγ2 | ND | 1.40 ± 0.09 (n = 12, P < 0.01) |
| IP-YFP + Go-CFP + Gβ1 + Gγ2 | 1.22 ± 0.09 (N = 16, NS) | ND |
| IP-YFP + Gs-CFP + Gβ1 + Gγ2 | 1.96 ± 0.14 (N = 13, P < 0.001) | 1.76 ± 0.09 (n = 20, P < 0.001) |
Statistical significance was calculated by using one-way ANOVA with a Bonferroni post hoc test using α2A-CFP + KCNQ1-YFP as the control conditions. The data shown for CFP + YFP and D2-CFP + M4-YFP (also indicated by *) is taken from Benians et al. (13).