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. 2005 Dec 12;102(51):18350–18355. doi: 10.1073/pnas.0506415102

Fig. 2.

Fig. 2.

ChIP assays detect La but not Ro at class III genes in vivo.(A) Anti-Ro can immunoprecipitate Ro-associated hY RNAs. Shown are Northern blots to detect U6 RNA and hY4 RNA after immunoprecipitation with anti-Ro anti-serum (lanes 1 and 2), anti-La antiserum (lanes 3 and 4), or nonimmune serum (lanes 5 and 6). Cells were either formaldehyde-treated (+ lanes) or mock-treated (–lanes) before harvesting. (B) ChIP assay using antibodies against TFIIB (lane 5), La (lane 6), pol III RPC155 subunit (lane 7), Ro (lane 8), Brf1 (lane 9), or no antibody control (lane 4). Primers specific to hY4 and hY5 were used to amplify the immunoprecipitated material.