Fig. 4.
Increased activity and expression of GALC in the shi spinal cord and their reduction after twi/CNP-EGFP OPC transplantation. (A) GALC activities of the excised dorsal column of the thoracolumbar spinal cord from twi, normal (MBPshi/+), shi, shi with dead twi/CNP-EGFP OPC transplantation (sham surgery), and shi with twi/CNP-EGFP OPC transplantation. Data are mean + SD (n = 3-6 in each group). (B) GALC immunolabeling of the dorsal spinal cord of twi, wild-type, shi, and the transplanted shi mice. (C) Optical density of GALC immunoreactivity in the dorsal column was expressed as relative intensity to the optical density for twi mice. Data are the mean + SD. (n = 3 in each group). (D) Confocal images of GALC immunolabeling (red) showed the localization of GALC in a twi/CNP-EGFP OL (arrows) in the graft. Reconstructed orthogonal images are presented as viewed in the x-z (Lower) and y-z (Right) planes. [Scale bars: 100 μm (B) and 10 μm (D).]