Fig. 4.

The differentiation of Lin^–CD34⁺dGFP^low and Lin^–CD34⁺dGFP^– EML cells transfected with HS2–2b-dGFP lentivirus. (A) The expression of dGFP in CD34⁺ or CD34^– EML cells differentiated from Lin^–CD34⁺dGFP^low EML cells. (a) The purification of the sorted Lin^–CD34⁺dGFP^low. (b–d) The sorted Lin^–CD34⁺dGFP^low cells were cultured in SCF medium for 1 day (b), 2 days (c), and 3 days (d). (B) The expression of dGFP in CD34⁺ or CD34^– EML cells differentiated from Lin^–CD34⁺dGFP^– EML cells. (a) The purification of the sorted Lin^–CD34⁺dGFP^–. (b–d) The sorted Lin^–CD34⁺dGFP^– cells were cultured in SCF medium for 1 day (b), 2 days (c), and 3 days (d).