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. Author manuscript; available in PMC: 2026 May 17.
Published in final edited form as: ACS Sens. 2026 Feb 6;11(2):909–922. doi: 10.1021/acssensors.5c01707

Figure 5.

Figure 5.

MDF differentiates receptor-specific TNFR1 and TNFR2 conformation changes in mixed cell populations. (A) R1 and R2 MDF biosensors show no change in FLT between single or mixed samples (two cell populations, each expressing one biosensor) for either D or DA biosensors. (B) R1 and R2 biosensor cells were treated with DMSO or 10 μM zafirlukast (Zaf) for 2 h. The TNFR1 small-molecule inhibitor Zaf disrupts R1 ΔFLT while having no change in R2 ΔFLT. (C) Similar to Zaf, a TNFR2-specific small molecule (drug-A treated at 10 μM for 2 h) reduces R2 DA FLT while having no effect on R1 or D only conditions. Data represent mean ± SD of n = 3 biological replicates; significance determined by one-way ANOVA (ns = p > 0.05; **** = p < 0.0001).