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. Author manuscript; available in PMC: 2026 May 17.
Published in final edited form as: ACS Sens. 2026 Feb 6;11(2):909–922. doi: 10.1021/acssensors.5c01707

Figure 6.

Figure 6.

MDF enables concurrent monitoring of two mechanistically different aSyn aggregation biosensors. (A) Schematic of aSyn-mScI3/aSyn-ShR single-fusion (aggregation) biosensor and the corresponding FLT for donor only and donor + acceptor (1:4 D:A ratio) conditions. (B) Schematic of te ShY-aSyn-mNg double fusion (folding) biosensor and corresponding FLT for donor only and donor + acceptor conditions. These aSyn MDF biosensors are being expressed in HEK293T cells. aSyn MDF biosensors under single and mixed cell biosensor conditions show no significant difference for either the oligomerization (C) or folding biosensor (D). Panels A-B generated in Biorender. Data represent mean ± SD of n = 3 biological replicates; significance determined by one-way ANOVA (ns = p > 0.05; **** = p < 0.0001).