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. Author manuscript; available in PMC: 2026 May 17.
Published in final edited form as: ACS Sens. 2026 Feb 6;11(2):909–922. doi: 10.1021/acssensors.5c01707

Figure 7.

Figure 7.

Utilizing MDF biosensors within the same cellular context. Co-expression of both red and green MDF control linker biosensors within the same cell (not-mixed cell populations) for the 532 nm channel (A) and 473 nm channel (B) shows no significant difference between donor-only and linker conditions, respectively. A similar true multiplexing of TNFR1 (R1) and TNFR2 (R2) MDF biosensors within the same cell (C) R1, 532 nm channel; (D) R2, 473 nm channel) showed no change between conditions (see Discussion section for details on transfection differences). Data represent mean ± SD of n = 3 biological replicates; significance determined by one-way ANOVA (ns = p > 0.05; ** = p < 0.01; **** = p < 0.0001).