Whole-mount in situ hybridization to mature S. mediterranea hermaphrodites using ESTs annotated to the GO term of reproduction. (A) Representative results of annotated ESTs expressed in testes (arrowheads). From the top left: PL06015A2C10, calmegin; PL03020B1B02, K+-channel modulatory factor 1; PL04019B2G03, cytoskeletal tropomyosin; PL06019A2G01, SMC3; PL06020B1C06, plastin 1; PL06020B1C11, bromodomain containing 2. (B) ESTs that are expressed in the ovaries (arrowheads), which can be viewed ventrally and are located posterior to the cephalic ganglia (asterisk). PL04006A1H09, mago nashi; PL06003X1E11, c14orf172. Detailed information for each homologue can be found in Table 7. Anterior is to the left in A and at the top in B. (Scale bars: A, 1 mm; B, 0.5 mm.)