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. 2005 Dec 27;4(1):e9. doi: 10.1371/journal.pbio.0040009

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) KIR2DL4 expression on the surface of NK cells immediately after isolation (day 0) or after 7, 14, or 21 d in culture with autologous feeder cells and rIL-2. Cells were stained with mAb 33.

(B) Resting NK cells were incubated with soluble (10 μg/ml), plate-coated (5 μg/0.1 ml/well), or bead-bound (4 beads/cell) antibodies to KIR2DL4 (mAb 33) or CD16 (mAb 3G8). After 24 h, culture supernatants were tested by ELISA for IFN-γ production.