Table 1.
Results of the Southern hybridizations of undigested plasmid preparations from various spiroplasma species and S. citri strains to either an arp1-derived probe or whole pBJS-O probe. + and - denote positive and negative hybridizations, respectively.
Spiroplasma | Probe | Biological features | |||
Species* | Strain | arp1 | pBJS-O | Transmissibility | Pathogenicity |
BR3-3X | + | + | + | + | |
BR3-G | + | + | - | - | |
BR3-T | + | + | + | + | |
S. citri | BR3-M | + | + | + | + |
BR3-P | + | + | Very low | - | |
ASP-1 | - | + | Unknown | Unknown | |
R8A2 | - | + | Unknown | Unknown | |
Beni Mellal | - | - | Unknown | Unknown | |
S. kunkelii | CR2-3X | + | + | + | + |
S. phoeniceum | P40 | - | - | +** | + |
S. melliferum | TS2 | + | + | Unknown | - |
S. floricola | 23-6 | - | - | Unknown | - |
*S. citri, S. kunkelii, S. phoeniceum and S. melliferum belong to serogroup I, whereas S. floricola belongs to serogroup III. The biological features of the spiroplasmas, except for S. phoeniceum, are taken from references 25 and 46.
**Only experimental transmission to the plant host is known for this spiroplasma. It is unclear whether it can be naturally transmitted by leafhoppers.