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. 2026 Apr 21;100(5):e00119-26. doi: 10.1128/jvi.00119-26

Fig 1.

Experimental data showing SHFL protein inhibits PRRSV infection through reduced nsp2-EGFP fluorescent expression, decreased nsp9 levels on Western blot, and dose-dependent reduction in virus titers with increasing SHFL concentrations.

SHFL overexpression inhibits PRRSV infection. BHK-21 cells were cotransfected with a PRRSV infectious cDNA clone (pCMV-TA-EGFP, 2 μg) and FLAG-tagged SHFL plasmids (0, 0.5, or 1 μg). (A) The infection efficiency, reflected by the expression of the nsp2-EGFP fusion protein, was assessed via fluorescence microscopy at 48 hpt (scale bar, 100 μm). (B) The expression of nsp9 and FLAG-tagged SHFL in BHK-21 cells was evaluated by Western blot analysis. β-Tubulin was detected as a loading control. (C) Culture supernatants harvested from the transfected BHK-21 cells at 48 hpt were used to infect MARC-145 cells, and virus titers were determined by the TCID50 assay. Data are presented as means ± SD of three replicates. Statistical significance was analyzed using a two-tailed unpaired Student’s t-test. **, P < 0.01.