Skip to main content
. 2026 Apr 21;100(5):e00119-26. doi: 10.1128/jvi.00119-26

Fig 10.

GLuc activity and protein expression analysis of PRRSV replicon with SHFL mutants. SHFL and 1-260 significantly inhibit viral replication as shown by reduced GLuc activity and nsp9 expression in HEK-293T and BHK-21 cells.

Identification of the key region essential for SHFL antiviral inhibitory effect on PRRSV. PRRSV replicon (1 μg) was cotransfected with SHFL or its mutant plasmids (0.5 μg) in HEK-293T and BHK-21 cells. At 24 hpt, cell supernatants were harvested for the GLuc assay (A and C) and cell lysates for Western blot (B and D) using an anti-FLAG antibody to detect SHFL mutant expression and an anti-nsp9 antibody to detect RNA replication. β-Tubulin was detected as a loading control. Data are presented as means ± SD from three replicates. Statistical significance was analyzed using the two-tailed unpaired Student’s t-test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, not significant.