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. 2026 Apr 21;100(5):e00119-26. doi: 10.1128/jvi.00119-26

Fig 4.

Experimental schematic and bar graphs showing SHFL knockdown effects on PRRSV lifecycle stages. Data reveal significant SHFL mRNA reduction with variable impacts on viral attachment, internalization, assembly, and release processes.

SHFL inhibits viral translation at the early stage of the PRRSV life cycle. (A) Schematic diagram of virus attachment, internalization, assembly, and release assays. (B and C) Effect of SHFL knockdown on the attachment of PRRSV. Cell-bound virions and SHFL expression levels were quantified by RT-qPCR. (D and E) Effect of SHFL knockdown on the internalization of PRRSV. Internalized virions and SHFL expression levels were quantified by RT-qPCR. (F) Effect of SHFL knockdown on the assembly of PRRSV. The packaging efficiency was calculated as the percentage of virion-associated genome in the total viral genome. (G) Effect of SHFL knockdown on the release of PRRSV. The release efficiency was calculated as the percentage of extracellular virions in the total virus yield. (B–G) Data are presented as means ± SD from three or six replicates. Statistical significance was analyzed using the two-tailed unpaired Student’s t-test. **, P < 0.01; ***, P < 0.001; ns, not significant.