TABLE 1.
Primers for plasmid construction
| Name | qPCR primer (5’−3’) |
|---|---|
| shfl-sgrna-F | AAAGGACGACGTCTCGGGTATCCTCCAAGAAGGCGGGTTTTAGAGCTAGAAATAGCA |
| shfl-sgrna-R | GCTATTTCTAGCTCTAAAACCCGCAGGGGTAAAGCAAAAACGAGACGTCGTCCTTTC |
| SHFL-F1 | CTGGCTAGCGTTTAAACTTA |
| SFLS-R1 | TGGTTGCGAAAGTTGTGCCTACACTTGGGGCA |
| SFLS-F2 | CACAACTTTCGCAACCACACACACAGCTGCTCC |
| SHFL-R3 | GTGCTGGATATCTGCAGA |
| SHFL-Zincmut-R1 | TCAGCGGAGCTGGCGGCAAACTGCCGGTCC |
| SHFL-Zincmut-F2 | CCGCCAGCTCCGCTGATCACGTGTGGTGGAGG |
| SHFL-Zincmut-R2 | CTAGCCTTTCTGGCCCGGGACACCTCCTTTCT |
| SHFL-Zincmut-F3 | GGGCCAGAAAGGCTAGGAAGCGGTACGAGC |
| SHFL-Δ102-105-F | CAGGAGGATGCCGAGTTCCACTGCCCCAAGTG |
| SHFL-Δ102-150-R | GAACTCGGCATCCTCCTGGGCTCTCTGGAACATC |
| SHFL-1-260-R | TGTGCTGGATATCTGCAGAATTCTCACAGGCCGCCCTGA |
| SHFL-R3-F | CCGCGTGCGCAAAGTGTGCGAAGCGGTACGAGCCAGTG |
| SHFL-R3-R | CTTCGCACACTTTGCGCACGCGGACACCTCCTTTCTCTG |
| luc-1PRF-78-F | GCAGTATTTCTACACCTCGAGGTGTTTAAACTGCTAGCCG |
| luc-1PRF-69-R | CATCTTCAATGGTGGCAGATCTTGTCTCAGTAACAACTAAGC |
| luc-1PRF-72-R | CATCTTCAATGGTGGCAGATCTCGCTGTCTCAGTAACAAC |
| luc-1PRF-75-R | CATCTTCAATGGTGGCAGATCTTACCGCTGTCTCAGTAAC |
| luc-1PRF-78-R | CATCTTCAATGGTGGCAGATCTTTTTACCGCTGTCTCAG |
| luc-1PRF-PN1-R | CATCTTCAATGGTGGCAGATCTTTTTACGGCAGTCTCAG |
| luc-1PRF-PN2-F | CACCTCGAGGTGTTTAAACTGCTAGCCGCCAGCGGCTTGACCGGCAGTGGTCGC |
| luc-1PRF-PN2-R | GGCAGATCTTTTTACGGCAGTCTCAGTAACAACTAAGCCGCCGCGACCACTGCCGGTCA |
| luc-1PRF-PN3-F | CACCTCGAGGTGTTTAAACTGCTAGCCGCCAGCGGCTTGACGGCGAACGGTCGC |
| luc-1PRF-PN3-R | GGCAGATCTTTTTACCGCTGTCTCAGTAACAACTAAGCCGCCGCGACCGTTCGCCGTCA |
| luc-1PRF-PN4-R | GGCAGATCTTTTTAGGCGAACCTCAGTAACAACTAAGCCGCCGCGACCGTTCGCCGTCA |
| luc-1PRF-R | CATCTTCAATGGTGGCAGATCTTGTCTCAGTAACAACTAAGC |
| luc-1PRF-IFC-F1 | GCAGTATTTCTACACCTCGAGGAAACTCCAGGGCCTGAC |
| luc-1PRF-IFC-R1 | AGCAGATTTAGACACTGCTCCTTAGTCAGGCC |
| luc-1PRF-IFC-F2 | GTGTCTAAATCTGCTAGCCGCCAGC |
| luc-1PRF-IFC-R2 | ATCTTCAATGGTGGCAGATCTGAATTTGACTATTTTTACCGCTG |
| sgdluc-2prf-F | AACCCCGGGCCCTACTGCCAGGTTTTTAGCCTCG |
| sgdluc-ifc-F | AACCCCGGGCCCTACTGCCAGGTATTCTTAGCCTCGTTTCCCAT |
| sgdluc-2prf-R | CTTATGCCGTGCCTCTCGGAGAATAACCACTGTCAG |
| P1650-F | GAGCTCTTACGCGTGCTAGCTCACTAATAGAGCAGGCTCC |
| P1000-F | GAGCTCTTACGCGTGCTAGCCCACAAAGAACCATCCAGC |
| P500-F | GAGCTCTTACGCGTGCTAGCCACCGCCCCCGCCAC |
| P200-F | GAGCTCTTACGCGTGCTAGCTGACCGACTGCAGAGGCCG |
| P100-F | GAGCTCTTACGCGTGCTAGCGCAGAGGCGGAGCCAAGG |
| Promoter-R | AGTACCGGAATGCCAAGCTTCTCCAGGCGGGCTCCGCC |
| B500-R | AGTACCGGAATGCCAAGCTTTGTGTTTGCCAGGATTCGAT |
| B200-R | TACCGGAATGCCAAGCTTCATGTCCCCACGCTGCG |
| B100-R | AGTACCGGAATGCCAAGCTTTCTTGCGCCCCAAACCCAG |