TABLE 3.
Primers and probes for RT-qPCR quantification
| Gene name | Primer (5′−3′) | Probe |
|---|---|---|
| PRRSV-M-qF | CGGCAARTGATAACCACGC | FAM-GTCGTCCGGCGTCCCGG-BHQ |
| PRRSV-M-qR | TGCCACCCAACACGAGG | |
| PRRSV-nsp9-qF | CGGCGGCTTAGTTGTTACTG | |
| PRRSV-nsp9-qR | TCAACCTCACTGGCCACTTT | |
| shfl-qF1 (MARC-145) | ATGAGGAAATTCGGCAGCGA | FAM-AGCGCCCAACTCCCGTGTGG-BHQ |
| shfl-qR (MARC-145) | ATGTCCCGGTCCTGTTTCAT | |
| shfl-F (HEK-293T) | CGGTTCCGCTGCCCTGAGGC | |
| shfl-R (HEK-293T) | GCTGCCGAATTTCCTCATCA | |
| actb-qF | CCCTGGAGAAGAGCTACGAG | HEX-CGGTTCCGCTGCCCTGAGGC-BHQ |
| actb-qR (MARC-145) | CAGGAAGGAAGGTTGGAAGAG | |
| actb-qR1 (HEK-293T) | GGAAGGAAGGCTGGAAGAGT |