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. 2026 Apr 13;33(5):756–767. doi: 10.1038/s41594-026-01788-6

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© The Author(s) 2026

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — Editing efficiency in K562 cells is shown for guides targeting AAVS1 (F1 and D4) and SOD1 (A9, D9, D10) that had previously displayed a range of editing efficiencies with a spacer length of 20 nt and a truncated version of the gRNA scaffold. Points represent the individual data (n = 2 independent biological replicates). Lines represent the mean value. Overall, guides with the 19 nt spacer displayed the highest levels of editing efficiency in this assay. The guide with the highest editing efficiency across all spacer lengths was AAVS1-F1. See Methods for further details. Source data are provided in Source Data file.

Source data