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. 2026 Apr 13;33(5):756–767. doi: 10.1038/s41594-026-01788-6

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© The Author(s) 2026

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 5 — a-g. Cryo-EM data analysis for OsCas12f (a), RhCas12f (b), OsCas12f intermediate state I (c), state II (d), state III (e), Al3Cas12f intermediate state I (f) and state II (g). Left to right panels show local resolution maps; gold-standard Fourier Shell Correlation (FSC) curves with resolution reported at FSC = 0.143; Euler angle distribution plots of particle orientations; map-to-model FSC curves.