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. 2026 Apr 13;33(5):756–767. doi: 10.1038/s41594-026-01788-6

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© The Author(s) 2026

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 7 — Interactions between the PAM duplex and OsCas12f (a), RhCas12f (b), and Al3Cas12f (c). d. Schematic of the GFP depletion assay. e-f. In vivo activity of wild-type (WT) and PAM-recognition mutants of OsCas12f (e) and RhCas12f (f). Data represent mean ± SD (n = 3 independent biological replicates). Significance determined by one-way ANOVA. Source data are provided in Source Data file.

Source data