Fig. 2. Cryo-EM structure of Cas12f orthologs reveal distinct dimerization interface and RuvC plasticity.

a–c, Domain organization, cryo-EM maps and models for Al3Cas12f (a), OsCas12f (b) and RhCas12f (c). Density is colored on the basis of proximity to the modeled domains. gRNA is colored red, REC domains are colored gold, WED domains are colored sandy brown, RuvC domains are colored sky blue and ZnF domains are colored royal blue. The DNA TS and NTS are colored green and pink, respectively.