Fig. 5. DNA target loading is facilitated by a two-lid opening mechanism.
a–c, Intermediate state structures illustrating conformational changes at three activation stages of OsCas12f: state I (a), state II (b) and state III (c). Active-site residues are shown in blue; the lid regions are shown in red. d, Structural alignment of intermediate state structures. The regions in the box and red circle are enlarged to illustrate the open-to-close conformational change of two lid regions (black arrow) and the position of the DNA substrate in the catalytic pocket. e, GFP depletion assays for lid mutations in OsCas12f. Data represent the mean ± SD (n = 3 independent biological replicates). Significance was determined using a one-way ANOVA.