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. 2026 Apr 13;33(5):756–767. doi: 10.1038/s41594-026-01788-6

Extended Data Fig. 2. Al3Cas12f protein purification.

Extended Data Fig. 2

a. S200i 10 300 SEC elution trace of Al3Cas12f (dark line) overlayed with elution trace of BioRad Gel Filtration Standards (dashed line, Bio-Rad Catalog #1511901). The Al3Cas12f peak runs elutes around 11.5 mL post-injection, similar to the second peak from the gel filtration standards (bovine γ-globulin, approx. 158,000 Da). b. SDS-PAGE gel of Al3Cas12f protein purification steps. Sample before (1) and after SEC (2-9). Samples 2–9 correspond to consecutive 0.5 mL fractions collected from elution volume 10 mL to 13.5 mL. M, Marker (Bio-Rad Catalog # 1610363).