Table 1.

Fit parameters of RTC assembly, elongation dynamics, and mechanochemistry model

Conditions
[nsp13] (nM)	0-50, 0, 20	–	–	–
F (pN)	20, 20–40, 20–40	–	–	–
tcut (s)	0.08	–	–	–
Value type	Best values	Mean values	SD values	Bounds
LL	−47,861	−47,4793	547	N/A
BIC	95,907	95,770	1,094	N/A
$\mathrm{\Delta }{G}_1\left(k_{\mathrm{B}}T\right)$	1.38	1.56	0.23	[−3, 3]
$\mathrm{\Delta }{G}_{\mathrm{1,2}}\left(k_{\mathrm{B}}T\right)$	0.90	0.77	0.17	[−3, 3]
$\mathrm{\Delta }{G}_{1,2^{\mathrm{*}}}\left(k_{\mathrm{B}}T\right)$	−0.72	−0.72	0.03	[−3, 3]
$k_{\text{post}}^{\text{FNA}}(1/s)$	97	104	5	[1, Inf]
$k_{\text{post}}^{\text{SNA}}(1/s)$	145	121	27	[1, Inf]
$k_{\text{pre,}0}^{\text{FNA.c}}(1/s)$	228	252	22	[1, Inf]
$k_{\text{pre,}0}^{\text{FNA.}2}(1/s)$	20	32	9	[1, Inf]
$k_{\text{pre,}0}^{\text{SNA.C}}(1/s)$	15,310	12,858	2,740	[1, Inf]
$k_{\text{pre,}0}^{\text{SNA.}2}(1/s)$	5,912	4,991	1,101	[1, Inf]
$k_{\text{in}}^{\text{SNA}}(1/s)$	3.9	3.9	0.1	[0, 10]
$k_{\text{in}}^{\text{VSNA}}(1/s)$	0.13	0.14	0.01	[0, 10]
$k_{\text{in}}^{\text{LLP}}(1/s)$	0.021	0.015	0.006	[0, 10]
$k_{\text{irr}}^{\text{FNA}}(1/s)$	40.1	39.9	0.4	[0, 100]
$k_{\text{irr}}^{\text{SNA}}(1/s)$	20.2	20.0	0.5	[0, 100]
$p_{\text{irr}}^{\text{VSNA.c}}$	1.00	0.92	0.05	[0, 1]
$p_{\text{irr}}^{\text{VSNA.}2}$	0.30	0.25	0.15	[0, 1]
$p_{\text{FNA}}^{\text{c}}\left(F_0\right)$	0.89	0.88	0.00	N/A
$p_{\text{FNA}}^2\left(F_0\right)$	0.95	0.95	0.00	N/A
$p_{\text{SNA}}^{\mathrm{c}}\left(F_0\right)$	0.086	0.087	0.002	N/A
$p_{\text{SNA}}^2\left(F_0\right)$	0.041	0.042	0.001	N/A
$p_{\text{VSNA}}^{\mathrm{c}}\left(F_0\right)$	0.022	0.022	0.001	N/A
$p_{\text{VSNA}}^2\left(F_0\right)$	0.0012	0.0011	0.0007	N/A
$p_{\text{LLP}}^{\text{c}}\left(F_0\right)$	0.0043	0.0049	0.0006	N/A
$p_{\text{LLP}}^2\left(F_0\right)$	0.0038	0.0040	0.0007	N/A
${\tau }_{\text{VFNA}}\left(F_0\right)\left(\mathrm{s}\right)$	0.02	0.02	0.00	N/A
${\tau }_{\text{FNA}}^{\mathrm{c}}\left(F_0\right)\left(\mathrm{s}\right)$	0.052	0.052	0.000	N/A
${\tau }_{\text{FNA}}^2\left(F_0\right)\left(s\right)$	0.036	0.037	0.000	N/A
${\tau }_{\text{SNA}}^{\mathrm{c}}\left(F_0\right)\left(s\right)$	1.57	1.59	0.04	N/A
${\tau }_{\text{SNA}}^2\left(F_0\right)\left(s\right)$	0.74	0.75	0.04	N/A
${\tau }_{\text{VSNA}}^{\mathrm{c}}\left(F_0\right)\left(s\right)$	4.3	4.4	0.0	N/A
${\tau }_{\text{VSNA}}^2\left(F_0\right)\left(s\right)$	4.8	4.8	0.0	N/A

t_cut, lower dwell-time cutoff; LL, log likelihood. See also Tables S2 and S3.