Table 2.
Analytical data for the studied methods.
| Validation parameter | Method I | Method II | |
|---|---|---|---|
| CAF | CAF | CUR | |
| Wavelength (nm) | 443 | 323 | 437 |
| Linearity range (ng/mL) | 30.0-800.0 | 50.0-2000.0 | 20.0-200.0 |
| Number of experiments (n) | 7 | 7 | 7 |
| Intercept (a) | 17.350 | 22.480 | 43.927 |
| Slope (b) | 1.065 | 0.353 | 3.711 |
| Correlation coefficient (r) | 0.9999 | 0.9999 | 0.9994 |
| Standard deviation of residuals (Sy/x) | 5.35 | 2.65 | 8.64 |
| Standard deviation of intercept (Sa) | 3.05 | 1.64 | 6.67 |
| Standard deviation of slope (Sb) | 7.0 × 10− 3 | 1.5 × 10− 3 | 5.7 × 10− 2 |
| %Relative standard deviation (%RSD) | 1.46 | 0.83 | 1.66 |
| %Error | 0.55 | 0.32 | 0.63 |
| LOD (ng/mL) | 9.44 | 15.40 | 5.94 |
| LOQ (ng/mL) | 28.60 | 46.66 | 17.98 |